Monday, April 19, 2010

micro focus stacking tests





This weekend I managed to start playing around with focus stacking, using Zerene Stacker. I seem to be getting mixed results from the final output images. There are however quite a few variables to play with, modifying depth of field through aperture size both on the camera and the microscope, varying number of images in a stack, light conditions, etc. The images above show the preliminary results. They were all imaged using the same aperture settings, about mid setting on the microscope and 6.3f on the camera. Each set of images contains the final stacked image on the right and two of many (40-50) images used to create the final stack, giving some sense of the obstacle of a reduced depth of field. These images were taken at roughly ~200x, ~1000x, and ~2000x magnification. It's neat to compare these images to those taken with the SEM in the gigapan below:

2 comments:

  1. BIOIMAGER has the largest collection of light microscopes and accessories in the world. Biological microscopes are available in both upright and inverted models with epi-fluorescence imaging capability. BIOIMAGER offers biological microscopes, metallurgical microscopes, stereo microscopes, polarizing microscopes, digital LCD microscopes with tablet cameras and digital cameras, live cell imaging chambers, confocal imaging dishes, and slide scanners as well as gel imaging. Our solutions include 2D imaging, 3D profile with output to 3D printer, 4D and even 5-Dimensional imaging with advanced image analysis software. Contact us: www.bioimager.com

    epi-fluorescence microscope
    industrial inspection microscope

    ReplyDelete

  2. Good job! I loved it and continue your passion.

    By the way, For more interesting and good quality of 3D printing, metallurgical microscope, and industrial inspection microscope.

    Visit our website : https://www.bioimager.com

    ReplyDelete